Phospholipase cocktail: A new degumming technique for crude soybean oil

Enzymatic degumming (EDG) is an emerging alternative process for decreasing the phosphorus content, increasing oil yield, and preserving quality. Purifine® 3G a cocktail of phospholipases composed phospholipase A2 (PLA2), C (PLC), phosphatidylinositol (PI-PLC). In this study, was applied to crude soybean oil, optimum conditions (enzyme concentration, temperature, water dosage) were determined using central composite rotatable design (CCRD). The contents diacylglycerols (DAGs) free fatty acids (FFAs) in studied system considerably increased at temperatures below 64 °C enzyme concentrations above 100 mg/kg, while content decreased with amount concentration. particular, EDG 200 mg/kg conducted 120 min temperature 60 concentration 3% (w/w) lowered residual 8.9 FFA DAG by 0.17% 0.72%, respectively. Meanwhile, retained tocopherol maintaining its Hence, increases neutral yield (FFA DAG), decreases preserves quality, which make it commercially viable agent.